Detailed Locus Information for CNAG_04036T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | ||||||||||||||||||
| Gene Symbol | HSF3 | ||||||||||||||||||
| TF Family Name | Winged helix repressor DNA-binding Classification based on Fungal Transcription Factor DB | ||||||||||||||||||
| KEGG Linkout | KEGG::CNB05180 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Functional Annotation |
|
||||||||||||||||||
| Amino Acid Sequence | MPAGNAGVRDGGLHISMEEGDMTPEMDPFYNPIMPPPQQSSKKKKARKTEGKQPTFLTKLYSLLSQPEYSHIIRWDEAGESIIIENPEELADKVLPVIYRQSRFASFSRQLNIYGFNRKLSLRNVEKGICDPDASSWSHPFLRRDSTKQEILSFKRRVPPRPSQAQKRRMSMGLGIGINPSSVGPPNREDQASPTSSERTLDWQSPPDPYQHHLLPDVDEEAPFVFPTRDYFGTAGYAPIAYQGWKHNDTVAGSVQGVMGQVEEQFSPTTSIHFDYGTPPDRDGLNGFMASSEVQHGESPKGLAISILRSSCPPNHHTQQQIQPSLLLPCQPASFSLLAQKSPMKLVPQSAPANTGSFPIPIRVTQQHVRTRSVQGELPSAMLFSPFGEELGEVPEPAGFSQSLNGTRGYRHQAGNMGPPAIPAAPTFDPSDPSTWARRGFIDLTTAVFSNPLPFNPVPTSARDATSPHSLPTSLSSLHKQQTASPSELMDQSMSSALSDDSPSTASPGIYQSGFSLPAYPLPSLQRHISSLNPPACTHSNSTANMSPNTGLGRSPHIARESNVRLSAIIQTKQDRRQSINASPYPYSAQSPRQRPVALNTSDSAGESGSWTGMNGGSLRTIGCSSRGSEGGNSAVGGSNVDGDERKQNGHLSL | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1126 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 190 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 218 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 281 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 305 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1145 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | CCAGTTGAACGCTTCTAAAC |
| L2 | 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTGATGGTGTGGGAACAATAC |
| PO | Southern blot probe primer | TCACAGATGCCTTTCTCG |
| R1 | 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTTCTCCTTGCCTATGTGCC |
| R2 | 3' flanking region primer 2 | CAGCAACTTACTTTGGAGGC |
| SO | diagnostic screening primer, pairing with B79 | ATAGACAGCAGCGGGATAC |
Phenotype Assays
| Growth | |
|---|---|
|
|
| Mating Data | |
|
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
|
|
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
|
|
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
|