Detailed Locus Information for CNAG_05535T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | |||||||||
| Gene Symbol | Unknown | |||||||||
| TF Family Name | Winged helix repressor DNA-binding Classification based on Fungal Transcription Factor DB | |||||||||
| KEGG Linkout | KEGG::CNH01860 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSHHPPAPSLAALPVDTSKTDPTPAYYKLQFGDDLTGFSYYVRTLTVVIGRNCERPAPVPPPAITSPSVPTNPPLDPNEPSPPGLCTGGILRTPPVASFNSPSVVEEDHEVNLEDYGPLVELAEAVAVKEEDVADDISPVLELPDAPPPPPPPKSSANVEHVDVDLGPLKSVSRNHAKIEYRSELGRFCLEILGRNGAWIDDRYYVKGAVVPLAQGSQIQIATRIFSFVLPPSSVPSPPYNYYDKSALENVENLPYPYNLPASEVRYQEFFGEAGPGPATAAAMASRQPPAFNAFAAADGYGLGLGVDVESQWGQWESEEESDSDVERDHEQSFERDSEKDAHEEEQAEDEDWDDAQSEDEDEDEYEEAKPKQATKIKLKKPLVVPGEDESDLSSLESEPAKPLKSVKKQAGKKQSQSQSKSVENEKVKAPKDAKKPSKKESKSKKKIKEPTPPVEESPAEVKGTPSKSKKKEKEKEKNKIDSLAEQKQEIQAVTSDEKEKATPQKKDQKKKIKPASESKPLVKPAPGLKLPIPLAQQQQQHQPATQPQPPSVPTLAAALANTPTPPTIAPTPTSQPVVRPPQQPVRPVPGQPQQGIVRPPGVSGQPGQPMIRPGQPGVPMGVRPPVSGSGQIPVRPGQPHSHLKQPLPSTPGVRPPLPQTPVPRTTPSSAGTTPQPTQPFFCTELKESSGRPGHIIVNVPIPPSGAGPRPPPGPILGLDGKPFIGPPPLKPTSTFATIIHKALSCLPRGRGTLGEVCNWVAGEWEWFRLNVDSGWQNSIRHNLSLNKAFLKVPRIPEDDPESKGSVWIIDPQEGPLFEEKQRRDAMKSASKDKNAESRREKERIRAEERAKKQREAAIEAARNPHAHPSYVHHPHPTMARAMPPMATARAIARPPANVAHPHPQPSTTQPVPNQPTSANIKGVLQPKAKITVVMQPITPALRAKSVIANTDSNGNPLPFVCDGTTLVLDQSTFGHLTSDILDKLTLLGAAGAVDVLSAWVINKNKMQASKAAQQAKTGATGTATTSGAAPGVVGAKNGVVNGAARPTNGVQPARPQPQVAPKPNTTPAARPAIPPKPAAASAASAAPTATVTTTPAQPAPSTTPAVKPASSNPPKSLPGPAPPGTSLTKVIGMIAAVANAKGDVNTVGPNASALLRYIRVVGVEIDLRVAERIWATGVVPPLPVKKVAKPVSGAATGSASVGGAKPTQGAGVKPQGQLQGGAKPMQNASVPAPAPSAAGVAGMKRKLEDGAQGQGLERGNSNGAGGGTGGDQEAKKPRLETSSV | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 3015 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 1365 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 1142 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 2267 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 2488 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 3246 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | AGCAACAACGGCATAGTG |
| L2 | 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACACGACAGTGAGAGTCCTGAC |
| PO | Southern blot probe primer | CATCGGCTACATCTTCCTC |
| R1 | 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCTGGAGCGAGGAAATAGTAATG |
| R2 | 3' flanking region primer 2 | ACACAAAGCGAGTGGTTG |
| SO | diagnostic screening primer, pairing with B79 | GGCAGTGTCAGATGAAAGC |