Detailed Locus Information for CNAG_04226T1
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | U4/U6.U5 tri-snRNP-associated protein 1 | ||||||||||||||||||
| Gene Symbol | SNU66 | ||||||||||||||||||
| TF Family Name | SART1 Classification based on Fungal Transcription Factor DB | ||||||||||||||||||
| KEGG Linkout | KEGG::CNI03670 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Functional Annotation |
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| Amino Acid Sequence | MQARDKEEQDRLMYGEEDLAGLKVGHGVEEFEEGEDVILTLKDTGVLEGGEDELQNVNLVEDAAIKAARERKRKAQQAYTGYDDEEFDENRIGQRADVLGKYDEDFATGKVRTEGFRLGAPVEMKMKIQDEDEQMGIAPARKVKLHLDYTKEFEVSDYAKEGEAGFKKPKKKKAKRSTRRTEAEEDTMEVDGESTFTRRVVTDGPDNLVDDEDLQAALSRARRANAKKKPKIKPEDVAAQIAQRKQEEGEQVKEEDGEEDGRITFDETSEFARNVSLESRAISVKRERISPPPSGITPVTEAGPSEEPVMVKIERREEGEVDDEDDEMGEEDADLAEIAAREGMSLEEYREKIDRQMREMEEMKKEAKDEDEAEPTVGQGVAGVLALLRHQGALKARTAEDEERERVQKQKDLWLADYRRRMAQRELERIQARGGNKDQAQREWENRMREQQEARDALDIYKNYKPDVNIVYHDEFGRQMTPKEAWKSLSHKFHGKTSGRMKTEKRLKKIAEERKQLTMNSSDTPLGMTDAFSRRQQMTGEAHMILSVGNKQSVQAGSSKRR | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 2975 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 3886 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 3711 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4564 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 4619 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 4523 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | GCCCTTTTTGGTATGACAG |
| L2 | 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTGTGAGGGATTCCTTGTTC |
| PO | Southern blot probe primer | ATACATCAGCCTGTCCTGC |
| R1 | 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGTTGGAAACAAGCAGTCG |
| R2 | 3' flanking region primer 2 | TCAGAAGAATCAAGAGGTCG |
| SO | diagnostic screening primer, pairing with B79 | ACTTACATTGCCTTCCTCG |