Detailed Locus Information for CNAG_06425T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
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| Gene Annotation | fungal specific transcription factor | |||||||||
| Gene Symbol | PPR1 | |||||||||
| TF Family Name | Zn2Cys6 Classification based on Fungal Transcription Factor DB | |||||||||
| KEGG Linkout | KEGG::CNN01490 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MKAPAEKLSKTKNYAPYDAASPKSLQPPLLPHKPKSNSSRPSQPPTPTDTTTDASGQSTYKVARAISSCTRCRSRKQKCDGKLPACSACERAKVECIGFDAISKTNISRNYLHQLEQEVTSLRAQVAALTSNDPIGRTKKGIAANASQALSAYRSDFPIDPSLPDDRSPRSSYDSSIPAGPSSYSSQHQRRLSDFPFSSTPANDSPRSTFAHTPNSRPHPTGQAASLHATSLTRLVHDAALRTGHATNGGPSLNPSGSNASSSDRGSVHGGVDSPILGIDREEPHNSPDMVLTPHSGHKRHPSASPLAASATLSVSSGGKPKRKIAIPPLPPQPAVERLVAAYVDFVGVTGPIIHIPTLGKQLLKIREGTDVEEGDIFVVMMVLALSTMASSRFVDPPDDLRACSEAFHAEAMKHLDAVFEEQSYVSLQAILLLVWYSLLNPDKGSIWFLVGLATRACVDLGYHNEHNVQVDQLDALELDMRRRLFWCTYKMDRLLSQSLGRPPSIPDGFVNVPLPSNLHDIDIHAGHYGPLTGEPCSYKAVFLHTTKLRQLQSEILFNTYGVHGSTGRLPSDEWFDDCYGRLKHWLKTAPEPRGTVSTEGFELSFHNSCLLLFRPSPGCPRPGRKALSTVLTSSGYIIRIYRRMQLNNRISWLWMTSHFSFMAGLSFLYAYFNLYSMGGGHDVPSIDDAMMDIESCLGVLEFLAPRVPSAAACHDTLRTLSQAIFKQLSDLDPPHTTVTSSPNRRSLLRSAPISSSREDPLPNVFPAPLPPVALPYELSLLDNLFRNPMASHNKASDYTSNKLCGKRKAEAEGYNQSTPLSMPSRAFHSAGEYPASQSSSGHGNGTISDPSTMMGTFGMAAHLNLHPGSNLPLFPPSSGPNLSTLANTAAAAAAAGSFTPAGEASNGAMLNVDNEQNGFDIFSFLMDDEGGLGMNGFSLDVPADFSLWG | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1044 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 1399 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 1345 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 1267 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 1230 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1885 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | CTTTCACGAACGCTTGATTC |
| L2 | 5' flanking region primer 2 | CTGGCCGTCGTTTTACTTGGACAGTTTTTCGGCGG |
| PO | Southern blot probe primer | CCCTTTTTTTGTGCGACC |
| R1 | 3' flanking region primer 1 | GTCATAGCTGTTTCCTGATGTGCCTGCGGATTTCTC |
| R2 | 3' flanking region primer 2 | TGAGACCGCTGAAAGTTCC |
| SO | diagnostic screening primer, pairing with B79 | TAATCGCCGACAAAGGGTG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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