Detailed Locus Information for CNAG_02700T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | hypothetical protein | |||||||||
| Gene Symbol | ZFC8 | |||||||||
| TF Family Name | C2H2 zinc finger Classification based on Fungal Transcription Factor DB | |||||||||
| KEGG Linkout | KEGG::CNK01880 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Functional Annotation |
|
|||||||||
| Amino Acid Sequence | MLTAFFGYVTELTCLVLLFTSFFLIPPPAPACTCECKCGRKNRIPAVFPSAGDASIKQSTTTEPESSKSVTDKEPRQPAKALEEQLSHPIEQQQQRGLKPPIGVETPQPIIPSIENAEKPIEFQKSTKSIEPIETPVKEEPLATPVPQDKESGWDASAPTERQIHSDPSVGGRFGDWEAEGEVNEKTVENNYDGQRNTEPFKSPTSRILDSLDDSTSSYRAVEDSQDGESLEYSRSPAKSETRSAISRPAFNGRPLPPHQVRSTDTFDHGYDYRSSIETSSYASKRKIELPVCKPCNKQFKSAAALQQHTQAVHSSPLFNKQSRSRMSSPEFSTHPPAPGSVTSSAGRPKSSFDDGFHSPYMCEICNEDYHSAYDLRQHKMEKHPWAMLCPECLVTFAHAHEARDHYELVHGSEPASVIRTQRMLDTIHSSTPSSRVPSASNSASNYAESTYATHAAKPPQYRILASYDSHVVQAEHRCQQCDMVFPNAMDLRAHVNSPFSHGGEKLTPDNFPPLGSTPVAPSSESVSQHNPIAPYASSSQPAWDTSMPSFVLAARLSSNRQRFNNAISPEAKLTPDDNPPEIEAEEAVPKLVETKAVVENTPAIEHEDVAREADEKVEDKNTEHERERQVELETETEIEQVHKHEQRSEHGLDVEPDYVATLEPVVADEHHCEVEMFDAAESPAAPEPIMKDRTGEAIQDEQSGQASQLEHGEQVHNHAQSSGSAIITGPETSSEPAPRDNTFKYNHPLPPQSKAAKKAMARQLYRNAPKQPKQPKKSKSKQKTQELFESDEDVDFELPSDFEQLTGQASEFGVSVTFIDEASRPSKKEINVQDDLASSNGLPKSLYSTARAKLDLPPATYQDESDEEEASGVVIEKEVTAMAPEPPTPAFDEEVIEIRRTIYIPRYDDYSDDEEKPEVDGASAEKQTPVIQSNQSKDATQEEEQKKDAQAPFTEPTVSSTFEAPIVTDMPEMVERLFVTDVNPADIASPEPLVHAATYEELPEKAYSSNTVAEEPSGPSSGLQQYQHFSETTMPSAAIEIIRPAEAVEGTKAADEVTAEQAPLPSDPAFAEIDAENDVRVEATSPSEAIAQSVEAFSGTEQDGLIGEAIEADTARQIDDDVEIQTTAESEATAAQPVEATNGTEHEGLIGEATEADASREVDDNAESKIPPELSQVDTSFTPAAPLQSLSHSSGLPYSRDEFAQPIATHDQASNLVDALQSNHPDNPKRSAVTHSPEQPAESMQIASSASPVMEGAGAGVAREEELAAIEAEEAARAAQALAEAEAEFAAAAAVAPAITEDEPSMIGNIEFSVPSFTFSESNTATITSAPLSLSTSEEHEEGTVEARTAESEQLIPSIAPADDDHWAESEEVYRIAIANRARPPLNLAPIRDRDSYEDDSRSFRFPPNRRINNHGPIRKRNSTGSNGWGPKPIPRPFKNSTNALEKMRSARAEVEDERAKDRERRKGTTSVRSSSFNGVDESGWISMDPKSYHNDRYGGSAGRYGDDGDSGWSPNDRQGSRQGWDDNRRASFSNSTTVSPPQNNNWGSLPGVADGDTGGW | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 8045 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 8125 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 8880 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 6789 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 9177 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 8274 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | TGAAATCAGACTCCAGCCTC |
| L2 | 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACGCGACCACATTTACACTCG |
| PO2 | Southern blot probe primer | AGAAAGCAGCATTTGGGG |
| R1 | 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGCCACAGAACAATAACTGGG |
| R2 | 3' flanking region primer 2 | GCGTATGAATAAGCGTTGG |
| SO1 | diagnostic screening primer, pairing with B79 | AGCAAAACAGAAGCAGCG |
Phenotype Assays
| Growth | |
|---|---|
|
|
| Mating Data | |
|
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
|
|
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
|
|
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
|