Detailed Locus Information for CNAG_05153T0
| Species | Cryptococcus neoformans var. grubii H99 |
|---|---|
| Gene Annotation | hypothetical protein |
| Gene Symbol | GAT5 |
| TF Family Name | HMG Classification based on Fungal Transcription Factor DB |
| KEGG Linkout | KEGG::CNL04700 |
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. |
| Functional Annotation | No functional annotation available. |
| Amino Acid Sequence | MSCPPIPNRIHQLPVRVSYYIPSSSQTYSTIFPSLQQVYIHPQSAGGDDETWGSIYLKTVVKGVLAASPELHPAYPGTSDLSLYVLDPRETYFRRARVGSAHHGNTDTAGSEVWTGKGLVSWSLAEHGQGKNLIAGRLERDVAFTTAIRTQGMSALEALMAADQMGNVGEESWGIDISLGLNMGLGAPGGFARARASSPAIQRRRSGLEKEESGQITPAYPRQQKRASVPSAPQTFDIRGELPAHPSSSSYSHDPPFRPSHNINHASSPIDPPRAHGRQTKSASTGGRPRKVTASSHSSSTSVDGADPRQRLAHSQRSYEIPLPSSEGPNVYDALSSIPENILARPESLTREQAQRLLASPAFIDMLGKITGTSIPTGKPNANNKRPRDGEHVEVQPPKRKRGRPTKAEKAAKEAQEAAARLAEAVKQKAEAVDGPQEDSRNPVCWNCGRTKSAIWRTKMMENGQSVRVCNACGLYWNKLGTMRPPNLWADGDDDRRSERAQSTFSEAPSEHGPAHRVDKPLARAQESFKRTLSAAVEQDARRMATRHKGRAPTSPNKHSKLGPMTSPPRGSASATKSLKQGKYAAASSPGGFMEAMHDSFESEVNEASPEDGNESPHFRARPAPHPHRLTRNPPSNTDTRTLDLPLSDDGLGSGAPHGNNEWNDEVSAFFDVEGFSMSTMVQHETPEYNRRHHGQEVIRETKHGAHRSNPIHVTSSSMMENEHVGTDSTLEEDTVLSQLFNRTSSIAGPGSSPSGFDFSQLPPSSPPMSVLSSDALPHSALLLSSPVKKNTPSVSGQTPNASGLTPADGNRLPQSSSKLRHSINAGDSHDNDRQAGGNGDIQQLDFEGIQRMFNLMSHPDDVSQKNTPAGTNHTPLSTFEDPQYGALNELIDGLNGDTSGMKMSVGVEMVCGGEGETSSASAVLADGNGEDIFASFLDGGAFV |
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1818 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 2735 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 3016 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 3074 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 2608 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 2816 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | 5' flanking region primer 1 | AAAACCAAGTGGAACCCG |
| L2 | 5' flanking region primer 2 | CTGGCCGTCGTTTTACTGGTTGACGCTGTTAGCAC |
| PO2 | Southern blot probe primer | CGGCGATAAGGTTTTTGC |
| R1 | 3' flanking region primer 1 | GTCATAGCTGTTTCCTGATAGGCAGGCTGGTGGAAAC |
| R2 | 3' flanking region primer 2 | ACCACATTTGCTTCCGAGC |
| SO1 | diagnostic screening primer, pairing with JOHE12579 | TGGCTGAACTGTAGGTTAGTG |
Phenotype Assays
| Growth | |
|---|---|
|
|
| Mating Data | |
|
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
|
|
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
|
|
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
|